|Name in Japanese||山崎 歩|
|Rōmaji Translation||Yamazaki Ayumu|
|Appears in||Anime, Manga, TV Drama, Stage Show|
|Voice Actor (Japanese)||Yūko Nagashima|
|Voice Actor (English)||Christine Auten|
|Relatives||Susumu Yamazaki (younger brother)|
Ayumu Yamazaki (山崎 歩 Yamazaki Ayumu ) is the "den mother" of the Shinsengumi. Ayumu (most often known as "Ayu-nee", or "big sister Ayu") is the cook and housekeeper at the Shinsengumi compound. A pretty woman with a motherly nature, Ayumu is fully cognizant of what the people around her do for a living, and acts as a sort of big-sister figure to Tetsu. She is Susumu's older sister, and, like him, a shinobi. She mentions in one episode that they are a part of the Oniwaban. She dies during the course of the anime.
- While Susumu is based on the actual spy, Ayumu is a fictional character.